A06 will investigate the role of neutrophils as drivers and modulators of the adaptive immune response in pemphigoid disease. It will characterize neutrophils in different organs and use the photo-convertible transgenic mouse line Kaede to understand their migration pattern in the early phase of the disease. Depletion of neutrophils and use of neutrophil-specific MHC-II knock-out mice will provide insights into their role as drivers and/or initiator of the adaptive immune response in PD. Finally, A06 will investigate the role of anaphylatoxin receptors C5aR1/2, which are essential for neutrophils in the effector phase, also in the afferent phase.